Table 1 Apparent kinetic parameters for laccase and Cu-containing artificial complexes with respect to the catalytic oxidation of a variety of reducing substrates
From: A supramolecular metalloenzyme possessing robust oxidase-mimetic catalytic function
kcat (s-1) | Km (mM) | kcat/Km (s-1 mM-1) | |
|---|---|---|---|
Fmoc-K/Cu2+ (2,4-DCP) | 0.0232±0.0060 | 5.1200±1.916 | 0.0045 |
GMP/Cu2+ (2,4-DCP) | 0.0010±0.0002 | 2.6660±0.7310 | 0.0004 |
Fmoc-K/GMP/Cu2+ (2,4-DCP) | 0.2236±0.0120 | 0.6962±0.1200 | 0.3211 |
Fmoc-H/GMP/Cu2+ (2,4-DCP) | 0.0751±0.0291 | 6.7900±3.0000 | 0.0111 |
Laccase (2,4-DCP) | 5.924±0.1266 | 0.2912±0.0314 | 20.6200 |
Fmoc-K/GMP/Cu2+ (2,4,6-TCP) | 1.0960±0.0880 | 0.2494±0.0540 | 4.6908 |
Laccase (2,4,6-TCP) | 33.7720±1.038 | 0.2677±0.0351 | 128.8610 |
Fmoc-K/GMP/Cu2+ (2,3,5,6-TCP) | 0.0800±0.0052 | 0.0683±0.0047 | 1.1826 |
Laccase (2,3,5,6-TCP) | 1.767±0.5949 | 0.0440±0.0032 | 41.3633 |
Fmoc-K/GMP/Cu2+ (TMB) | 1.3150±0.0270 | 0.8018±0.1150 | 1.6800 |
Laccase (TMB) | 8.3038±1.7595 | 0.2374±0.1092 | 48.7215 |
Fmoc-K/GMP/Cu2+ (DTBC) | 1.1960±0.0370 | 0.4530±0.0150 | 2.4247 |
Laccase (DTBC) | 5.3164±0.3418 | 1.417±0.1370 | 3.1801 |
Fmoc-K/GMP/Cu2+ (DMP) | 2.9500±0.8820 | 19.5700±8.4800 | 0.1507 |
Laccase (DMP) | 15.6835±0.6139 | 0.0229±0.0029 | 684.2785 |
