Fig. 8: The function of Ptbp2 in axon growth is dependent on hnRNP R.

a Representative images of control and Ptbp2-depleted motoneurons expressing hnRNP R-EGFP (sh1Ptbp2+hnRNP R-EGFP) at DIV 7 immunostained for EGFP and Ptbp2. Scale bar, 10 µm and 5 µm (magnified areas). The images are representative of three biological replicates. b Representative images of control and Ptbp2-depleted motoneurons, and of Ptbp2-depleted motoneurons expressing hnRNP R-EGFP at DIV 7. Motoneurons were immunostained against tau and EGFP. Scale bar, 50 µm. c SuperPlots of axon length measurements. One-way ANOVA with Tukey’s multiple comparisons test. Data are mean ± s.d. of n = 3 biological replicates. Representative images (d) and SuperPlots (e) of growth cone sizes of control and Ptbp2 knockdown motoneurons, and of Ptbp2-depleted motoneurons expressing hnRNP R-EGFP at DIV 6. Motoneurons were stained with phalloidin. Scale bar, 5 µm. One-way ANOVA with Tukey’s multiple comparisons test. Data are mean ± s.d. of n = 3 biological replicates. f Tau immunostaining of cultured Hnrnpr^+/+ and Hnrnpr^-/- motoneurons at DIV 6. Scale bar, 50 µm. g SuperPlots of axon lengths. One-way ANOVA with Tukey’s multiple comparisons test. Data are mean ± s.d. of n = 3 biological replicates. h Immunoblot analysis of Ptbp2 and hnRNP R in Hnrnpr^+/+ and Hnrnpr^−/− motoneurons transduced with control lentivirus, and in Hnrnpr^+/+ and Hnrnpr^-/- motoneurons transduced with lentiviruses for Ptbp2 knockdown or overexpression. The immunoblots are representative of three biological replicates. Source data are provided as a Source Data file.