Table 1 Stability of recombinant ToNV crystals
From: Atomic structure of a nudivirus occlusion body protein determined from a 70-year-old crystal sample
Stress type | Condition | Duration (h) | Diffraction |
|---|---|---|---|
Chaotropic agent | 8 M Urea | 24 | Yes |
Detergent | 10% w/v SDS | 24 | Dissolved |
Acid | 1 M HCl | 24 | No |
Base | 20 mM NaH2CO3 pH 10.5 | 3 or 24 | Dissolved |
Solvent | 100% Ethanol | 3 or 24 | Yes |
High molarity | 4 M NaCl | 24 | Yes |
Oxidising agent | 1% v/v H2O2 | 24 | Yes |
Reducing agent | 10 mM Dithiothreitol | 24 | Yes |
Physical stress | 90 °C temperature | 3 | Yes |
Freeze-thaw | – | Yes |
