Fig. 6: JTALP001 antibody promoted osteoblast mineralization.
a–c scFv-Fc (JTALP001) promoted hMSCs mineralization. Alizarin red staining and Von Kossa staining of hMSCs at different concentrations of JTALP001 after 14 days of osteogenic induction (left). Quantification of the percentage of Alizarin red (+) area and Von Kossa (+) area (right). n = 3 biologically independent samples. d The phosphatase activity of cell lysates from hMSCs treated with osteogenic medium for 7 days measured by phosphatase substrate assay. n = 3 biologically independent samples. e–h JTALP001 boosted TNAP induced osteoblast mineralization. Alizarin red staining and Von Kossa staining after differentiation of Alpl−/− pre-osteoblasts for 21 days in the presence of wild TNAP, TNAPS368A, TNAPR152H, TNAPK264R, TNAPR450C, or TNAPT167M proteins with further administration of 100 nM Fc fragment as control or 100 nM scFv-Fc (JTALP001). Quantification of the percentage of Alizarin red (+) area and Von Kossa (+) area. n = 3 biologically independent samples. All data in this figure are represented as mean ± SD. One-way ANOVA with Tukey’s multiple comparisons test for (b, c, f, h). All experiments were repeated three times independently with similar results. Source data are provided as a Source Data file.
