Fig. 2: Lipid nanoparticles deliver mRNA encoding the N-terminal domain of GSDMB into cells to induce pyroptosis. | Nature Communications

Fig. 2: Lipid nanoparticles deliver mRNA encoding the N-terminal domain of GSDMB into cells to induce pyroptosis.

From: mRNA lipid nanoparticle-mediated pyroptosis sensitizes immunologically cold tumors to checkpoint immunotherapy

Fig. 2

a Cell morphologies of the treated HEK 293, HeLa, 4T1 and B16F10 cells were detected using a confocal microscope. Before imaging, cells were treated with annexin V-FITC and propidium iodide (PI) and incubated for 15 min. Scale bars = 20 μm. Data are representative of three independent experiments. b LDH release-based cell death assay in HEK 293, HeLa, 4T1, and B16F10 cells after treatment with naked GSDMBNT mRNA, LNPs, or GSDMBNT mRNA@LNPs, respectively. Data are presented as means ± SD (n = 3). Statistical significance was calculated via one-way ANOVA. c Flow-cytometry analysis of cells positive for propidium iodide and annexin V. Data are presented as means ± SD (n = 3). Statistical significance was calculated using a two-tailed Student’s t test. Untreated cells served as the control (Ctrl) in all experiments. Source data are provided as a Source Data file.

Back to article page