Fig. 3: GSDMBNT mRNA@LNPs induce immunogenic pyroptosis and stimulate the maturation of bone marrow-derived dendritic cells (BMDCs). | Nature Communications

Fig. 3: GSDMBNT mRNA@LNPs induce immunogenic pyroptosis and stimulate the maturation of bone marrow-derived dendritic cells (BMDCs).

From: mRNA lipid nanoparticle-mediated pyroptosis sensitizes immunologically cold tumors to checkpoint immunotherapy

Fig. 3

a A confocal microscope was used to detect CRT expression in treated HEK 293, HeLa, 4T1, and B16F10 cells. Scale bar = 20 μm. Data are representative of three independent experiments. b, c Extracellular HMGB1 and ATP expression were analyzed by ELISA in HEK 293, HeLa, 4T1, and B16F10 cells after different treatments. Data in b are presented as means ± SD (n = 3). Data in c are presented as means ± SD (n = 4). Statistical significance was calculated using a two-tailed Student’s t test. d, e Immune stimulation of BMDCs by GSDMBNT mRNA@LNPs. B16F10 cells were pretreated with PBS (Ctrl), naked GSDMBNT mRNA, blank LNPs or GSDMBNT mRNA@LNPs, followed by coculture with BMDCs for 48 h. d Quantitative determination of proinflammatory cytokines IFN-γ, IL-1β and TNF-α using ELISA assay. Data are presented as means ± SD (n = 3). Statistical significance was calculated via one-way ANOVA. e Analysis of DC maturation biomarkers (MHC-II and CD86) using flow cytometry (n = 3). Source data are provided as a Source Data file.

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