Fig. 8: Local combinational treatment of GSDMBNT mRNA@LNPs and aPD-1 controls tumor burden at distant sites. | Nature Communications

Fig. 8: Local combinational treatment of GSDMBNT mRNA@LNPs and aPD-1 controls tumor burden at distant sites.

From: mRNA lipid nanoparticle-mediated pyroptosis sensitizes immunologically cold tumors to checkpoint immunotherapy

Fig. 8

C57BL/6 mice (n = 4 mice per group) were inoculated subcutaneously (s.c.) with 5 × 105 and 2.5 × 105 B16F10 cells in the left and right flanks, respectively. a Experimental timeline for treatment of B16F10 dual-tumor-bearing mice. b In vivo bioluminescence images of luciferase signals in mice treated as indicated for monitoring tumor growth. Imaging was performed every 5 days from the initial treatment day (day 7 after tumor inoculation) until day 23. c Individual curves of luciferase signals for mice treated as indicated. d, e Representative images and quantitative analysis of immunofluorescence staining for CD8+ T cell infiltration and CRT expression in tumors after indicated treatments. Scale bar = 50 μm. Results are presented as means ± SD (n = 5). Statistical significance was calculated using a two-tailed Student’s t test. Source data are provided as a Source Data file. Panel a is created with BioRender.com.

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