Fig. 7: IS transposition into cas genes in the presence of NHEJ.
From: Insertion sequence transposition inactivates CRISPR-Cas immunity
a Schematic diagram showing the assembly of the three NHEJ systems, which include the ku and ligD genes involved in NHEJ repair. b Transposition frequency was assayed in MG1655-ΔrecA harboring ompF, the target gene for DSBs mediated by SpCas9-HF1/sgRNA, followed by error-prone NHEJ DNA repair. Addition of l-arabinose (10 mM) induced ku expression. Up to 45 surviving colonies per plate were randomly selected for PCR validation, and the proportion of IS insertions into the SpCas9-HF1 coding region was calculated for each condition. Graph shows means ± S.D. derived from three biological replicates. ns, not significant; ***P < 0.001, using two-tailed unpaired t-test; P = 0.822, 0.000119, 3.3e-05 (from left to right). Source data are provided as a Source Data file.
