Fig. 2: Ι Expression of PfCRT variants in X. laevis oocytes.
a Confocal fluorescence images of fixed water-injected oocytes and PfCRTDd2 and E207A-expressing oocytes. Left, fluorescence image of Alexa 633 conjugated wheat germ agglutinin (WGA) as a marker of the oolemma. Middle, fluorescence image of PfCRT, using a specific rabbit antiserum and an anti-rabbit Alexa Fluor 546 secondary antibody. Right, differential interference contrast image (DIC). Scale bar, 135 μm. Representative images of 4 biologically independent samples. b Western analysis of total protein lysates from oocytes injected with water, PfCRTDd2-RNA, or E207A-RNA, using a polyclonal guinea pig antiserum specific to PfCRT and a polyclonal rabbit antiserum specific to α-tubulin. Representative images of 4 biologically independent samples. A size standard is indicated in kilodaltons. The uncropped images are presented in Supplementary Fig. 18. c Quantification of the PfCRT-specific signal strengths from 4 biologically independent samples (data points), normalized to the internal standard α-tubulin. A box plot analysis was overlaid over the individual data points, with the median (black line), mean (red lines), and 25% and 75% quartile ranges being shown. The whiskers above and below the box indicate the 90th and 10th percentile. The two datasets were not significantly different, according to the two-tailed Student’s t-test. Source data are provided as a Source Data file86.
