Fig. 4: Ι Effect of pH on PfCRT-mediated CQ transport kinetics.
a CQ transport kinetics of PfCRTDd2 at different proton concentrations (pH 4.5, 5.0, 5.5, and 6.0). Each data point represents the mean ± SEM of at least 3 biologically independent samples (range 3 to 14). b Sixteen different models of inhibition were fitted to the data represented in (a) using the least-squares methods. Plausibility was analyzed by calculating the Akaike information criterion difference (ΔAICc) and the Akaike weight. Models are shown in descending order. The kinetic parameters derived from the two most plausible inhibition models are presented in Table 1. c Double reciprocal Lineweaver-Burk Plot obtained with the data described in (a). d CQ transport kinetics of PfCRTDd2 and E207A at a pH of 4.5 and 6.0. Note, the E207A variant exhibits comparable CQ transport kinetics at pH 4.5 and 6.0. Each data point represents the mean ± SEM of at least 3 biologically independent samples (range 3 to 6). See Table 2 for kinetic parameters. Source data are provided as a Source Data file86.
