Table 1 Kinetic parameters describing interactions of PfCRTDd2 with CQ and protons

From: pH-dependence of the Plasmodium falciparum chloroquine resistance transporter is linked to the transport cycle

parameter

kinetic model

 

noncompetitive (partial)

mixed (partial)

 

mean ± SEM

95% CI

mean ± SEM

95% CI

Vmax (pmol h−1 oocyte−1)

40 ± 4

30.53–44.51

45 ± 9

28.1–61.75

KSCQ (µM)

220 ± 25

170–268

320 ± 100

123–517

KSH+ (µM)

1.80 ± 0.60

0.61–2.98

2.54 ± 1.08

0.43–4.66

α

  

0.53 ± 0.26

0.02–1.04

β

0.36 ± 0.02

0.31–0.40

0.27 ± 0.06

0.15–0.40

  1. Vmax, the maximum velocity of substrate transport, KsCQ and KsH+, the dissociation constants for CQ-PfCRTDd2 and H+-PfCRTDd2 complexes, respectively; α, the factor by which these KS values change when the other substrate is already bound to the transporter; β, the factor by which the Vmax is affected by the inhibitor; CQ, chloroquine, H+, protons. CI, confidence interval. Shown are the best-fit values of kinetic parameters obtained using the partial noncompetitive and partial mixed-type inhibition models.
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