Fig. 4: BID1 is required for the secretion pathway in nodule cells.
From: A signal peptide peptidase is required for ER-symbiosome proximal association and protein secretion
a, d and g Secretion of NCR001, NCR166 and CAML1 were blocked in bid1 nodule cells. GFP-fused NCR001, NCR166 and CAML1 were expressed under the BID1 promoter in WT and bid1, respectively. 14-day-old nodules inoculated with S. meliloti Rm1021 mCherry were analyzed by confocal microscopy. Bar=10 μm. b, e and h GFP-fused NCR001, NCR166 and CAML1 could not be detected in bid1 symbiosome. Symbiosomes were isolated from nodules in a, c, and e respectively, and analyzed by confocal microscopy. Bar=5 μm. c and f, In Western blot assay of isolated symbiosome, secretion of NCR001 and NCR166 were blocked in bid1 nodule cells. GFP-fused NCR001, NCR166 were expressed under BID1 promoter in WT and bid1. Symbiosomes were isolated from 14-day-old nodules inoculated with ABS7 hemA::LacZ. Protein samples were subjected to Western blot. Protein amounts loaded in each sample were labeled. Experiments were repeated more than 3 times with similar results.
