Fig. 4: Profiling the thermodynamics of DNA-binder interactions using BIND.
From: Enabling programmable dynamic DNA chemistry using small-molecule DNA binders
a Numerical values of ΔG, ΔH, and −TΔS for 15 DNA binders using BIND. These values were ranked against −TΔS. b Comparing values of enthalpy contribution obtained using BIND and values reported in the literature for 12 binders with previously reported thermodynamic parameters. Pearson correlation analysis was used to determine the confidence interval with P = 0.05 (n = 12), which was highlighted in gray shading. c Determining ΔH and ΔS of a representative intercalator, thiazole orange, by plotting lnKa against reaction temperature and fitting using Van’t Hoff’s equation. d Predicted binding mode of thiazole orange using molecular docking. e Determining ΔH and ΔS of a representative minor groove binder, Hoechst 33258, by plotting lnKa against reaction temperature and fitting using Van’t Hoff’s equation. f Predicted binding mode of Hoechst 33258 using molecular docking. g Determining ΔH and ΔS of netropsin by plotting lnKa against reaction temperature and fitting using Van’t Hoff’s equation. h Predicted binding mode of netropsin using molecular docking.
