Fig. 4: Intracellular segment.
Intracellular segment of HcKCR1 (a) and HcCCR (b) colored in magenta and blue, respectively, with the cavities colored in yellow and green. Key residues are displayed as stick models. Water molecules are represented by spheres. The black dashed lines indicate H-bonds. The internal cavities modelled with HOLLOW50 are shown. The backbone of TM 2 is omitted for clarity. c The reversal potentials (Vrev) of channel currents measured in the variants with Ile, Ser or Thr at the residue position 73. Wild-type HcKCR1 contains Ile, and wild-type HcCCR, Ser at this position. The symbols (black for the I mutants, red for the S mutants and blue for the T mutants; filled for the peak currents and empty for the end currents) are the mean values, the error bars, s.e.m. (n = 8 cells for HcKCR1_I73S and WT HcCCR, and 7 cells for other variants). p = 2.97E-07 (peak currents) and 5.20E-09 (end currents) by one-way ANOVA with the Tukey correction for multiple comparisons (alpha 0.0001 for HcKCR1 currents and 0.05 for HcCCR currents) to the respective I variants. n.s., not significant. Source data are provided as a Source Data file.
