Fig. 6: Extracellular aromatic cluster.
Extracellular regions of HcKCR1 (a), HcCCR (b), and bacteriorhodopsin (c, PDB ID: 1C3W) are colored in magenta, blue, and green, respectively, with the cavities colored in yellow, green, and grey. Key residues lining the cavities are displayed as stick models. Presence of Y222 instead of T222 in HcKCR1 occludes the channel pore compared to HcCCR. Cavities were calculated with the program HOLLOW50. The reversal potentials (Vrev) of channel currents measured in the mutants of extracellular residues in HcKCR1 (d) and HcCCR (e). The symbols (colors indicate individual mutants; filled for the peak currents and empty for the end currents) are the mean values, the error bars, s.e.m (n = 8 cells for WT HcCCR, and 7 cells for other variants). p = 3.21E-33 (HcKCR1 peak currents), 3.12E-33 (HcKCR1 end currents), 2.82E-01 (HcCCR peak currents) and 0.59 (HcCCR end currents) by one-way ANOVA with the Tukey means comparison to the respective WTs. n.s., not significant. Source data are provided as a Source Data file.
