Table 1 Cryo-EM data collection, refinement, and validation statistics
HcKCR1 | HcCCR | |
|---|---|---|
Data collection and processing | ||
Microscope | FEI Titan Krios | FEI Titan Krios |
Detector | Falcon 4i | Falcon 4i |
Magnification (Å) | 75,000 | 75,000 |
Voltage (kV) | 300 | 300 |
Electron exposure (e−/Å2) | 40 | 40 |
Defocus range (μm) | 0.8–2.4 | 0.8–2.4 |
Pixel size (Å) | 1.03 | 1.03 |
Symmetry imposed | C3 | C3 |
Initial particle images (no.) | 2,685,194 | 2,674,318 |
Final particle images (no.) | 297,015 | 296,840 |
Map resolution (Å) | 2.88 | 2.84 |
FSC threshold | 0.143 | 0.143 |
Map resolution range (Å) | 2.4–3.3 | 2.4–3.2 |
Refinement | ||
Initial model used | ab initio | ab initio |
Map sharpening B factor (Å2) | −158.5 | −153.1 |
Model composition (monomer) | ||
Nonhydrogen atoms | 2330 | 2284 |
Protein residues | 240 | 240 |
Lipids | 8 | 9 |
Water | 15 | 15 |
B factors (Å2) | ||
Protein | 65.80 | 58.81 |
Lipids | 71.57 | 62.23 |
Water | 68.73 | 54.52 |
R.m.s. deviations | ||
Bond lengths (Å) | 0.003 | 0.003 |
Bond angles (°) | 0.449 | 0.447 |
Validation | ||
MolProbity score | 1.06 | 1.20 |
Clashscore | 2.50 | 1.73 |
Poor rotamers (%) | 0 | 0 |
Ramachandran plot | ||
Favored (%) | 97.90 | 95.38 |
Allowed (%) | 2.10 | 4.62 |
Disallowed (%) | 0 | 0 |
