Fig. 3: Inhibiting HCK activity and HCK KO decreased macrophage proliferation, and migration in BMDM and Raw264.7. | Nature Communications

Fig. 3: Inhibiting HCK activity and HCK KO decreased macrophage proliferation, and migration in BMDM and Raw264.7.

From: HCK induces macrophage activation to promote renal inflammation and fibrosis via suppression of autophagy

Fig. 3

A BMDM cells of WT and HCK KO and dasatinib treatment were tested with MTT assay for cell proliferation. B Representative image and quantification of propidium Iodide (PI) stain detected with flow cytometric to measure cell cycle for WT and HCK KO BMDM. C Click-iT™ Edu cell proliferation assay and Ki67 IF staining were performed to measure WT and HCK KO BMDM proliferating. D Scratch assay was performed in BMDM cells for WT and HCK KO to measure cell migration. The blue lines are the scratch edge of the cells generated by the software when to measure the scratch distance. E 3D transwell assay with Matrigel® Matrix to measure cell migration for WT and HCK KO in BMDMs. F 3D-random migration analysis was performed with long time live cell imaging for BMDMs with WT, HCK KO and dasatinib treatment. (A–E) *p < 0.05. Source data are provided as a Source Data file.

Back to article page