Fig. 3: Structural and mutagenesis analysis of SulE. | Nature Communications

Fig. 3: Structural and mutagenesis analysis of SulE.

From: Crystal structures of herbicide-detoxifying esterase reveal a lid loop affecting substrate binding and activity

Fig. 3

a–g The substrate binding pocket of S209A/H333A with MM, EM, TrM, CE, SM, TM, and BM, respectively. Residues (Ile43 and Tyr45) belong to the lid loop of another subunit are shown in light pink. Arg150 is displayed in deep salmon. Other residues involved in substrate binding are shown in white. The seven sulfonylureas are also highlighted in different colors. Hydrogen bonds are shown in black dashed lines. The red dotted line indicates the distance. h Superposition of the seven substrates. i The relative activity of WT SulE and its variants to MM. Data were presented as mean values ± SD, n = 3. Error bars represent the standard deviation from three repeats. ND not detected. Statistical analysis was performed by the two-tailed t-test. *p = 0.0020. Source data are provided as a Source Data file.

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