Fig. 7: Amino acid mutation analysis on lid loop. | Nature Communications

Fig. 7: Amino acid mutation analysis on lid loop.

From: Crystal structures of herbicide-detoxifying esterase reveal a lid loop affecting substrate binding and activity

Fig. 7

a Effects of glycine and proline mutations in the lid loop on enzyme activity. b Effects of substitution of Pro44 by hydrophilic amino acids on enzyme activity. The relative enzyme activity of the variants to MM. Data are presented as mean values ± SD, n = 3. Error bars represent the standard deviation from three repeats. An unpaired two-tailed t-test was used to determine the statistical significance. *p = 0.0039 (G34A), *p = 0.016 (G49A), **p = 0.00013 (P33G), ***p = 4.8E − 06 (P38G), ***p = 1.7E − 05 (P41G), ***p = 6.3E − 05 (P44R), ***p = 1.3E − 05 (P44N), *p = 0.0020 (P44Q), **p = 0.00036 (P44K), ***p = 3.2E − 05 (P44S), and ***p = 2.0E − 05 (P44T). ns no significance. Source data are provided as a Source Data file.

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