Fig. 1: Induction of glomerular disease in AGMs by SARS-CoV-2 2019-nCov inoculation.
From: SuPAR mediates viral response proteinuria by rapidly changing podocyte function
a Experimental scheme for SARS-Cov-2 2019-nCov inoculation (n = 4 per group, 2 male, 2 female). Age and gender-matched control African green monkeys (AGMs) were either mock-infected with culture media used for virus propagation (n = 1) or untreated (n = 3 biological replicates). The illustration of AGM was created with BioRender.com. b Plasma soluble urokinase receptor (suPAR) levels in 2019-nCov inoculated AGMs upon nephropsy (n = 4 per group). P = 0.0034, two-tailed t test. c Proteinuria in AGMs upon nephropsy (n = 4 per group). Alb, albumin; Creat, creatinine. P = 0.011, two-tailed t test. d Renal histological examination, Periodic acid-Schiff (PAS) staining. Black scale bar, 100 μm. e Renal histological examination, Sirius red staining. Black scale bar, 150 μm. d, e The experiments were repeated four times, generating similar results. f Maximum intensity projection images obtained with podocyte exact morphology measurement procedure (PEMP), indicating the disorganized slit diaphgram in “2019-nCov”. White scale bar, 2 μm. g Calculated filtration slit density (FSD) that reflects the extent of foot process effacement (n = 30 replicates for control, n = 60 replicates for 2019-nCov). The analysis was conducted with two-sided Mann–Whitney test. b, c, g Data were presented as Mean ± SD. *P < 0.05, **P < 0.01, ****P < 0.0001.
