Fig. 1: The PEDOT-S derivative A5 was used as an in vivo injectable conductive polymer.
From: In situ assembly of bioresorbable organic bioelectronics in the brain
a Chemical structure of A5 and the two ETE-R trimers ETE-S and ETE-PC. b Time-lapse following the microinjection of A5 into the tailfin of an albino zebrafish. Images were obtained from the same fish after 1 h, 1 week, 2 weeks, and 3 weeks. n = 3 fish. c Workflow describing A5 microinjection into live zebrafish brains, including brain excision and cryosectioning. d Microscopy images depicting brain slices with part of the polymer track as observed in zebrafish that were left to swim for 2 h or 9 days after injection; bright-field image to the left with fluorescence image of the same slice showing the RedOx staining, where the magenta shows inflammation from the initial injection, but not from the polymer (2 h: n = 6 fish; 9 days: n = 9 fish). e Electrical measurements of brain slices placed on interdigitated Au electrodes. Au electrode numbers are indicated. n = 3 fish. f Current–voltage sweeps obtained between the contacts shown in the figure. Scale bars: 1 mm (b), 100 µm (d), 500 µm (e), 100 µm (f ). Figure created with BioRender.com.
