Fig. 1: Ezh2 is translocated to the cytoplasm in cardiac M2-like immunomodulatory macrophages in vivo.

Representative pictures of cardiac immunostaining for Ezh2 in a Cd11b⁺/Cd68^- monocytes, b Cd68⁺/iNos⁺, c Cd68⁺/Cd86⁺, d Cd68⁺/MHCII⁺ pro-inflammatory differentiated macrophages, e Cd68⁺/Cd206⁺ immunomodulatory differentiated macrophages at 24 h after coronary ligation in mice to induce MI or f Cd68⁺/Cd206⁺, g Cd68⁺/Lyve1⁺ cardiac-resident immunomodulatory in healthy sham mice. Nuclei were stained with DAPI (blue), myeloid markers were Cd11b (red, panel a), Cd68 (green, panels a–g), iNos (red, panel b), Cd86 (red, panel c), MHCII (red, panel d) Cd206 (red, panels e and f) and Lyve1 (red, panel g). Ezh2 (yellow) cellular localization was observed in each cell type but only appeared in immunomodulatory macrophages cytoplasm. Arrows indicate monocytes (white), non-determined macrophages (yellow), pro-inflammatory (green) and immunomodulatory macrophages (red), scale bars represent 25 μm. These cardiac immunostainings have been reproduced at least on three different mice with similar results.