Fig. 3: Stoichiometry of Hermes/LE-DNA transpososomes. | Nature Communications

Fig. 3: Stoichiometry of Hermes/LE-DNA transpososomes.

From: Zinc-finger BED domains drive the formation of the active Hermes transpososome by asymmetric DNA binding

Fig. 3

a Mass photometry data of the purified Hermes transposase dialyzed against three different buffers containing 0.5, 0.25, and 0.15 M NaCl. The theoretical mass of a Hermes monomer, dimer, hexamer, and octamer are 70, 140, 421, and 561 kDa, respectively. b Mass photometry data of the Hermes/LE-DNA complexes at 0.15 M NaCl. The sequences of the top strand of the double-stranded DNAs are presented on top. The bases in gray were absent on the top strand, but their complementary bases were present on the bottom strand. The subterminal repeats STR1 to STR4 are underlined and their orientation is indicated by arrows. The Hermes-to-DNA ratio is given on top of each plot. The fitted masses of P1 and P2 are reported on top of each plot, and their molecular composition (Hermes:DNA) are reported inside each plot. The theoretical MW of a Hermes hexamer and octamer are 421 and 561 kDa, respectively.

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