Fig. 6: Spindlin1-HBx regulates HBV transcription with reprogrammed cccDNA chromatin modifications. | Nature Communications

Fig. 6: Spindlin1-HBx regulates HBV transcription with reprogrammed cccDNA chromatin modifications.

From: Molecular insights into Spindlin1-HBx interplay and its impact on HBV transcription from cccDNA minichromosome

Fig. 6

a–c HepG2-NTCP cells were infected with normalized amount of HBV WT or HBV X-. At 7dpi, cells were harvested and analyzed by ChIP using antibodies against H3K4me3 (a) and H3K9me3 (b), and HBV RNA levels were quantified by Northern blot (c). d–h Effect of depletion of Spindlin1 (d) on enrichment of H3K4me3 and H3K9me3 at HBV cccDNA (e, f) and rDNA (g, h) in HBV infected HepG2-NTCP cells. (a–b, e–h) Input and immunoprecipitated DNA were analyzed in triplicate by qPCR with primers specific for cccDNA or rDNA and were displayed as percentage of input. As a control, immunoprecipitation was performed with rabbit IgG. Data represent the mean ± SD (n = 3 independent experiments). P-values between the groups were calculated with an unpaired two-tailed t test. ns, not significant difference. Source data are provided as a Source Data file.

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