Fig. 1: FAP106 is required for parasite motility.
From: FAP106 is an interaction hub for assembling microtubule inner proteins at the cilium inner junction
29-13 (Ctrl) and FAP106 knockdown (FAP106 KD) parasites were grown in the presence of tetracycline to induce knockdown. A qRT-PCR analysis of FAP106 mRNA levels. Graph shows the mean ± standard deviation from three independent biological replicates. Unpaired, two-tailed t-test **** p value ≤ 0.0001. B Cumulative growth curve shows the mean cell density ± standard deviation vs. time from three independent biological replicates. C Histogram shows the length distribution of purified, demembranated flagella. D Histogram shows the distribution of paraflagellar rod (PFR) lengths as measured by anti-PFR immunofluorescence microscopy in detergent-extracted cytoskeletons. E Graph shows the mean ± standard deviation of flagellum lengths in (C) (Phase Contrast) and (D) (anti-PFR). Phase contrast: Ctrl = 22.0 ± 2.5 µm, FAP106 KD = 19.0 ± 3.1 µm; anti-PFR: Ctrl = 17.6 ± 2.7 µm, FAP106 KD = 14.2 ± 3.2 µm. N = 50 flagella for each. Unpaired, two-tailed t-test ****p < 0.0001. F Motility tracks of individual parasites. G Mean squared displacement (MSD) of parasites tracked in (F). Dotted lines indicate the upper and lower bounds of the standard error of the mean. Ctrl N = 489; FAP106 KD N = 675. Data from an independent biological experiment are shown in Supplementary Fig. 1. Source data are provided as a Source Data file.
