Fig. 4: MC8 is a lineage-specific T. brucei MIP that comprises the MIP B8 density.
From: FAP106 is an interaction hub for assembling microtubule inner proteins at the cilium inner junction
A AlphaFold model for MC847,48, shown as ribbons and colored according to confidence. Box indicates high-confidence region modeled in (B). B MC8 AlphaFold model (ribbon) fitted into MIP B8 cryoET density (gray). (Left) Cross-sectional view of the DMT cryoET density map, with the MIP B8 density colored aqua. (Middle, Right) Enlarged view of MIP B8 density with AlphaFold model shown in aqua. Viewing angle shown in Right panel is indicated by the dashed line in Middle panel. C–E Demembranated flagella were purified from 29-13 (Ctrl) and MC8 knockdown (MC8 KD) parasites grown in tetracycline to induce knockdown. Structure of the 48-nm repeat of the DMT was obtained by cryoET, followed by sub-tomographic averaging. C Cross-sectional view of the DMT with MIP B8 density colored in aqua. D, E Longitudinal views of the DMT. Viewing angles and colors are as indicated in Fig. 2C, D.
