Fig. 4: RBM39 interacts with spliceosomal components.
From: Molecular basis of RNA-binding and autoregulation by the cancer-associated splicing factor RBM39
a Immunoprecipitation from HeLa nuclear extracts performed using an anti-Y12 antibody to pull down all Sm-class snRNPs. The western blots were probed with anti-RBM39 and anti-U1C antibodies (n = 3). b Immunoprecipitation from HeLa nuclear extracts performed using an anti-U1A antibody to pull down U1 snRNP. The western blots were probed with anti-RBM39 and anti-U1C antibodies (n = 3). c Immunoprecipitations from HeLa nuclear extracts performed using an anti-RBM39 antibody in absence or presence of RNAse and Cyanase. Western blots were probed with antibodies against SF3A3 and U1-A (n = 3). d FLAG immunoprecipitation of different FLAG-RBM39 constructs upon transient transfection in HeLa cells. Western blots were probed using anti-SF3A3 and anti-U1-C antibodies (n = 3). e Barplot showing the ratio between the western blot signal intensities of SF3A3 versus FLAG in the IP fractions. Average values and standard deviations of three biological replicates (n = 3) are shown. P values were computed using two-sided t test.
