Table 1 Thermodynamic analysis of the protein–RNA and protein–protein interactions
From: Molecular basis of RNA-binding and autoregulation by the cancer-associated splicing factor RBM39
Protein | RNA | Kd (μM) | n | ΔH (kcal/mol) | −TΔS (kcal/mol) |
|---|---|---|---|---|---|
RBM39 RRM1 | SL3 | 15 ± 3 | 0.95 | −136 | 108 |
RBM39 RRM1 | SL4 | 11 ± 4 | 0.78 | −68 | 40 |
RBM39 mRRM1.1 | SL3 | >100 | n.d. | n.d. | n.d. |
RBM39 mRRM1.2 | SL3 | >100 | n.d. | n.d. | n.d. |
RBM39 RRM2 | AGCUUUG | 9 ± 2 | 1.18 | −8 | −0.8 |
RBM39 RRM2 F259A | AGCUUUG | 41 ± 10 | 1.27 | −0.5 | 0.9 |
RBM39 RRM2 H258A/F259A/R289A | AGCUUUG | >100 | n.d. | n.d. | n.d. |
RBM39 mRRM2 | AGCUUUG | >100 | n.d. | n.d. | n.d. |
RBM39 RRM12 | ssSL3 | 0.6 | 0.93 | −333 | 297 |
RBM39 RRM12 | SLi | 8 ± 3 | 0.86 | −89 | 60.6 |
Protein | Peptide | Kd (μM) | n | ΔH (kcal/mol) | −TΔS (kcal/mol) |
RBM39 RRM3 | KSRWDETP | 19 ± 5 | 0.65 | −20 | 13.6 |
RBM39 mRRM3 | KSRWDETP | >100 | n.d. | n.d. | n.d. |
