Fig. 1: CITE-seq characterization of CD31+ cells from E10.5 AGM.
From: Meis1 establishes the pre-hemogenic endothelial state prior to Runx1 expression
A UMAP representation of single-cell unsupervised clustering based on transcriptome data. B Genes associated with each cluster in (A) that identify cell types. C, D Expression of antibody-derived tag (ADT) for (C) each cluster previously identified represented by ridge plots and D scatter plots of the relevant subpopulations. E Heatmap of surface expression, based on ADT, of the selected markers on cells forming the EHT cluster. F Identification of phenotypic HE within the “EHT’ subcluster on a UMAP projection. Cells labeled in blue are negative for CD117, CD41, CD43, and CD45 based on ADT expression and red cells expressed at least one of the EHT markers.
