Fig. 2: Identification of Meis1 as candidate regulator of early hemogenic commitment.
From: Meis1 establishes the pre-hemogenic endothelial state prior to Runx1 expression
A UMAP representation of the aEC, preHE, and EHT clusters only and schematic showing the number of DEG between each cell population along the transition. B Heatmap showing expression of the 12 TFs enriched in pre-HE cells compared to aEC, ranked by significance (Wilcoxon rank sum test (two-sided) with Bonferroni correction for adjusted p-values). C Selected GO terms associated with the 12 TFs identified in B (hypergeometric p-value with g:SCS correction using the default settings in gprofiler2). D Co-occurrence of the 12 TFs in B with the 425 pre-HE enriched genes, based on EnrichR analysis (p-values based one-sided Fisher’s exact test with Benjamini-Hochberg correction). E Overlapping genes between our Meis1 ChIP-seq dataset, published ChIP-seq datasets, and genes enriched in pre-HE vs aEC in the CITE-seq data. F Selected GO terms associated with pre-HE enriched genes categorized based on the presence of Meis1 binding sites in ChIP-seq data (hypergeometric p-value with g:SCS correction using the default settings in gprofiler2).
