Table 1 Overview of the amyloid-like APR crystal structures
APR | Polymorph | PDB ID | Crystallization conditions | Backbone conformer(s)a | Co-crystallising reagent(s) | Amyloid classb | Shifted assembly modec |
|---|---|---|---|---|---|---|---|
DFINWL (GLP-2) | A | 8ANJ | 70% H2O / 30% ACN, 0.1% TFA, 37 °C at acidic conditions (pH: 2–3) | A1 (skyblue) | H2O | class 1 parallel | Slight lateral shift between every second sheet |
pEFIAWL (GLP-1) | A | 8ANK | 70% H2O / 30% ACN, 0.1% TFA, 37 °C at acidic conditions (pH: 2–3) | A1 (deepsalmon) | H2O | class 7 antiparallel | No shift |
Ac-EFIAWL (GLP-1) | A | 8ONQ | 0.1 M citrate buffer, (pH: 4.0) | A1 (aquamarine) A2 (deepblue) | H2O | class 7 antiparallel | Lateral shift between sheets |
LFIEWL (exendin-4) | A | 8ANN | 70% H2O / 30% ACN, 0.1% TFA, 37 °C at acidic conditions (pH: 2–3) | A1 (greencyan) | H2O | class 8 antiparallel | No shift |
B | 8ANL | H2O, 37 °C, pH: 5.5 | B1 (green) B2 (limon) B3 (darkyellow) B4 (forestgreen) | H2O | class 7 / 8 antiparallel | Lateral shift between sheets | |
LYIQWL (Tc5b, E19) | A | 8ANH | 66% H2O / 33% ACN, 0,1% TFA, 4 °C, at acidic conditions (pH: 2–3) | A1 (lightpurple) A2 (deeppurple) | H2O, ACN, TFA | class 8 antiparallel | Shifted along the β-spine axis |
B | 8ANM | H2O, 37 °C, at isoelectric point of the peptide (pH: 5.6) | B1 (teal) | H2O | class 8 antiparallel | Lateral shift between sheets | |
C | 8ANI | 90% H2O / 10% EtOH, 37 °C at acidic conditions (pH: 3–5) | C1 (orange) C2 (olive) | H2O, EtOH, TFA | class 8 antiparallel | Shifted along the β-spine axis | |
D | 8ANG | 70% H2O / 30% EtOH, 37 °C, at acidic or neutral conditions (pH: 4–7) | D1 (red) | EtOH | class 1 / 4 parallel | Lateral shift between sheets |
