Table 1 Relative (Sub)MDF performance of random specificities compared to the wild-type specificity and number of necessary cofactor swaps (starting from the wild-type specificity) to reach the theoretically optimal (Sub)MDF obtained with the flexible specificity
From: Network-wide thermodynamic constraints shape NAD(P)H cofactor specificity of biochemical reactions
Oxygen availability | Growth rate [h−1] | Percentage of random specificities with higher/equal/lower (Sub)MDF compared to the wild-type specificity | Number of necessary swaps in wild type to reach (Sub)MDF of flexible specificity | ||
|---|---|---|---|---|---|
MDF | SubMDF | MDF | SubMDF | ||
Aerobic | 0.868 | 0.1%/0%/99.9% | 0%/0%/100% | 6 | 2 |
Aerobic | 0.818 | 0%/0.4%/ 99.6% | 0%/0%/100% | 0 | 3 |
Aerobic | 0.768 | 0%/2.3%/ 97.7% | 0%/0%/100% | 0 | 2 |
Aerobic | 0.718 | 0%/5.6%/ 94.4% | 0%/0%/100% | 0 | 2 |
Aerobic | 0.668 | 0%/11.8%/ 88.2% | 0%/0%/100% | 0 | 0 |
Aerobic | 0.618 | 0%/14.1%/ 85.9% | 0%/0%/100% | 0 | 0 |
Aerobic | 0.568…0.518 | 0%/14.9%/ 85.1% | 0%/0%/100% | 0 | 0 |
Aerobic | 0.468…0.118 | 0%/15.0%/ 85.0% | 0%/0%/100% | 0 | 0 |
Aerobic | 0.068 | 0%/17.0%/ 83.0% | 0%/0%/100% | 0 | 0 |
Aerobic | 0.05 | 0%/17.0%/ 83.0% | 0%/0%/100% | 0 | 0 |
Anaerobic | 0.371 | 7.9%/0.3%/ 91.8% | 13.4%/0.4%/86.2% | 1 | 1 |
Anaerobic | 0.321 | 0%/8.7%/ 91.3% | 0%/7.1%/92.9% | 0 | 0 |
Anaerobic | 0.271 | 3.7%/0.4%/ 95.9% | 1.8%/0.3%/97.9% | 9 | 14 |
Anaerobic | 0.221 | 0%/0.6%/ 99.4% | 0%/0%/100% | 0 | 2 |
Anaerobic | 0.171 | 0%/0.6%/ 99.4% | 0%/0%/100% | 0 | 5 |
Anaerobic | 0.121 | 0%/0.6%/ 99.4% | 0%/0%/100% | 0 | 4 |
Anaerobic | 0.071 | 0%/2.3%/ 97.7% | 0%/0%/100% | 0 | 3 |
Anaerobic | 0.05 | 0%/2.3%/ 97.7% | 0%/0%/100% | 0 | 3 |
