Fig. 5: NBPXa and DBPα are closely localised prior to egress but exhibit distinct localisations upon secretion. | Nature Communications

Fig. 5: NBPXa and DBPα are closely localised prior to egress but exhibit distinct localisations upon secretion.

From: Sequential roles for red blood cell binding proteins enable phased commitment to invasion for malaria parasites

Fig. 5

a Image of mNeonGreen (mNG) tagged NBPXa and DBPα schizonts. Scale bars = 5 μm. b Immunofluorescence assay showing HA tagged NBPXa, AMA-1, and RON2 (red), detected in schizonts using an anti-HA antibody. DBPα-mNG detected in same parasites (green) with an anti-mNG antibody. White boxes depict regions enlarged for ‘inset’ panels. c Extended depth of focus images of NBPXa-mNG and DBPα-mNG in post egress merozoites. Scale bars for (b) and (c) = 2 μm.

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