Fig. 7: Single-nuclei RNA-sequencing of developing mammalian face demonstrates conservation of cell types across species.

a, a’ Co-projected UMAPs of CS17 and E12.5 craniofacial tissues of (a), all cell types (a’), cells from “Mesenchyme” cluster in (a). Combined UMAPs were generated by subsetting matrices to only include genes with 1:1 orthologs across species. Generated via Seurat¹⁴⁵. b, b’ Co-projected UMAPs as in a, a’, overlaid with the species identity of each cell (red = human, blue = mouse). c, c’ Dot plots of gene expression for canonical cell type marker genes in each of the major cell types for all cell types, (c) and mesenchymal cells (c’). Size correlates to the percent of cells per cluster which express each gene. Color corresponds to the average expression of that gene within cells of that cluster (high expression=blue). d, d’ Module scores of hub genes from selected WGCNA modules calculated across major cell types via Seurat (ModuleScore). Color indicates average expression based on combinatorial expression from all genes of each module, across cells within each cluster (low = blue, high = red). Percent expression is indicated by size.