Fig. 3: Deletion of CD83 in microglia exacerbates autoimmune neuroinflammation.

a Schematic depiction of conditional knockout strategy. b Verification of successful deletion: microglia were isolated 7 weeks after tamoxifen injection via FACS. After RNA isolation, Cd83 gene expression was analyzed in CD83^ΔMG cells in relation to controls (n = 12, pooled from three independent experiments). c, d Gene expression analysis of different gene transcripts in acutely isolated microglia. Expression levels were first normalized to Rpl4 and relative expression was calculated in relation to controls (n = 12, pooled from three independent experiments). e EAE course over 30 days, comparison between CD83^ΔMG and CD83wt^Cre controls. Evaluation of the individual peak score of each mouse and the cumulative score, calculated from the area under the curve (AUC) of each mouse. (n = 15 for CD83wt^Cre and n = 17 for CD83^ΔMG, data are pooled from three independent experiments). f Gating strategy of CNS-infiltrating and resident immune cells (pre-gated on CD45⁺ cells) isolated at the peak of disease (day 18 p.i.). g, h Relative percentage of microglia, monocytes, monocyte-derived cells (MDCs), and monocyte-derived dendritic cells (moDCs) among all CD45⁺ cells at the peak of disease (n = 18 for CD83wt^Cre, and n = 19 for CD83^ΔMG, data are pooled from three independent experiments). i expression of Ccl2, Ccl4, and Ccl5 on on day 18 p.i. (n = 12 for CD83wt^Cre, and n = 13 for CD83^ΔMG, data are pooled from two independent experiments). In all graphs, data are represented as mean ± SEM and two-tailed Mann–Whitney U-test was used to analyze the data.