Fig. 1: VdUlpB is required for pathogenicity of V. dahliae. | Nature Communications

Fig. 1: VdUlpB is required for pathogenicity of V. dahliae.

From: DeSUMOylation of a Verticillium dahliae enolase facilitates virulence by derepressing the expression of the effector VdSCP8

Fig. 1

a Confirmation of the knockout mutant VdΔulpb by Southern blotting. Genomic DNA was digested with EcoR I at 37 °C overnight. The probes were labeled with biotin. b Colony morphology of wild-type (WT) V592, VdUlpB knockout mutant VdΔulpb and complementary strains, VdΔulpb/UlpB-1 and VdΔulpb/UlpBm-1, with WT VdUlpB or SUMO-specific protease site mutated VdUlpBm, on PDA plates after 19 days post-incubation (dpi). c Disease symptoms of cotton plants infected with the indicated strains at 22 dpi. d The disease grades were divided into five levels of disease symptoms severity in cotton leaves. Disease grades were evaluated with three replicates of 36 plants for each inoculum (mean ± s.d., t test, two-sided). e Identification of complementation of VdΔulpb with WT VdUlpB (full-length of VdUlpB: 1063aa) or mutated VdUlpB (VdUlpBm) by western blotting with anti-HA antibody, and Ponceau staining served as a loading control. The experiments in a, e were repeated independently three times with similar results. Source data are provided as a Source Data file.

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