Fig. 2: The landscapes of BC-associated DNA methylation signatures in PBMCs by Infinium 850 K microarray.

a The volcano plot for differentially methylated CpG positions (DMPs) between BC patients and normal controls. The x-axis is the mean DNA-methylation difference (delta Beta), and the y-axis is the log10 of the P value. Hyper-methylated CGs are shown in orange, hypomethylated CGs are shown in blue, and non-significant methylated CGs are shown in gray. b Bar charts representing the relative distribution of DMPs. Orange and blue bars represent hypermethylation and hypomethylation events, respectively. c Distribution of upstream and downstream methylation of TSS in PBMCs of BC patients and normal controls. P values were determined by two-sided loess regression. TSS, transcription start site. d The GO enrichment results of differentially methylated genes showed that multiple items were involved in the immune system and immune surveillance system. The size of the dot represents the number of differential genes, the color of the dot represents whether the q value is significant, and the trait of the dot represents different GO classification. e Unsupervised hierarchical heatmap clustering of eight candidate methylation markers that are differentially methylated between BC samples (n = 50) and normal controls (n = 30). Source data are provided as a Source Data file.