Fig. 4: Amikacin binding site at inter-subunit bridge B5.
From: Molecular basis of the pleiotropic effects by the antibiotic amikacin on the ribosome
a Simplified representation of the 70S ribosome with the AMK binding site indicated with the green star. b The unbiased (Fo – Fc) difference electron density map of AMK bound at the inter-subunit space is contoured at 2.3σ. c AMK interacts with elements of inter-subunit bridge B5, helix h44 of 16S rRNA, helix H64 of 23S rRNA, and ribosomal protein uL14. The amine group of the AHB group forms water-mediated (cyan) H-bonds with the phosphate backbone of h44.
