Fig. 8: Proteomic analysis of B. theta OM under B12-restrictive (0.4 nM CNCbl) vs. B12-replete conditions (40 nM CNCbl.).

a Relative abundance of OM proteins calculated using iBAQ intensities for cells grown in B12-restrictive conditions. b Volcano plot showing the magnitude of change in the expression of OM proteins between B12-restrictive vs. B12-permissive conditions. Note that several proteins from the three loci are not detected under at least one condition. c Heatmap showing the LFQ intensities in both conditions, with each square representing an independent sample. Five independent cultures were grown in minimal media supplemented with 0.4 or 40 nM CNCbl for 18 h in anaerobic conditions. Grey indicates the protein has not been detected in this condition.