Fig. 3: T cell response post-MVA immunization. | Nature Communications

Fig. 3: T cell response post-MVA immunization.

From: Intradermal but not intramuscular modified vaccinia Ankara immunizations protect against intravaginal tier2 simian-human immunodeficiency virus challenges in female macaques

Fig. 3

a Germinal center Tfh response (CXCR5++PD1++) (at MVA-1**p = 0.008, at MVA-2 *p = 0.031) and b germinal center B-cell response (BCL6 + Ki67+) (**p = 0.008) measured on CD4 + T cells and CD20 + B cells respectively in FNAs processed draining inguinal lymph node. The shaded area represents the range of germinal center responses at pre-vaccination time points from MVA-ID animals. Data were not available for the MVA-IM group. Left: representative flow-cytometer plots post-MVA immunization. c Circulating non-Tfh response (CXCR5-Ki67+) and Tfh response (CXCR5 + Ki67+) (***p = 0.0007) measured on blood CD4 + T cells (frozen PBMC) post-MVA-1 immunization. Left: representative flow-cytometer plots. d Th1(CXCR5- CXCR3+) /Tfh(CXCR5 + CXCR3-) in blood Ki67 + CD4 + T cells (frozen PBMC) (*p = 0.034). Left: Representative flow cytometer plots. Asterisks in all the above data, denote statistically significant differences measured by Mann–Whitney rank-sum (two-tailed). e Total SHIV BG505 (Gag + Env) specific IFN-γ + CD4 T cell responses and CD8 T cell responses measured in blood. Boxes in all the box plots extend from the 25th percentile to the 75th percentile of the dataset and the line inside the box denotes the median. The whiskers outside the box descend to the dataset’s minimum values and ascend to the maximum values. f Kaplan–Meier curves in animals with low and high frequencies of total SHIV BG505 (Gag + Env) specific IFN-γ + CD4 T cells (all vaccinated animals included and stratified as low and high, based on the median frequency as cut off) post-MVA-1 immunization compared with control animals. Statistical significance by log-rank test (*p = 0.028). g–i Spearman’s correlation (two-sided) between Day 7 MVA-1 induced IFN-γ + CD4 T cells and g MVA-1, Day 7 Th1/Tfh ratio, h Wk68 vaginal IgA (ng anti-SOSIP IgA per µg total IgA), i Wk70, serum ADP score. In all the correlation plots, p and r values were color-coded to represent the vaccine group (red: MVA-ID, Blue: MVA-IM, Black: both groups combined). These values were not shown for the group that didn’t have a statistically significant p value <0.05. DNA-1: first DNA; DNA-2: second DNA; MVA-1: first MVA; MVA-2: second MVA; Prt-1: first protein; Prt-2: second protein. Source data are provided as a Source Data file.

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