Fig. 1: Intensity of mKO1 native-fluorescence and number of mKO1-positive neurons at sites of monkey cortical injections of AAV2, AAV2.1-A, AAV2.1-B, and AAV1 vectors. | Nature Communications

Fig. 1: Intensity of mKO1 native-fluorescence and number of mKO1-positive neurons at sites of monkey cortical injections of AAV2, AAV2.1-A, AAV2.1-B, and AAV1 vectors.

From: A mosaic adeno-associated virus vector as a versatile tool that exhibits high levels of transgene expression and neuron specificity in primate brain

Fig. 1

a Schematic diagrams showing the injection loci of eight distinct types of recombinant AAV vectors carrying the mKO1 gene in the medial wall of the frontal lobe in Monkey A (upper) and Monkey B (lower). Scale bars, 5 mm. b mKO1 native-fluorescence (red) and NeuN immunofluorescence (green) at each vector injection site. Representative images are taken from Monkey A. Similar images were also obtained in Monkey B. Scale bar, 1 mm. c Comparison of mKO1 native-fluorescence intensity within a 2-mm-diameter circular observation window. Data are expressed as the averaged value obtained in Monkeys A (filled symbols) and B (open symbols), relative to the mean for the AAV2-CMV vector defined as 1.0. d Comparison of mKO1-positive neuron number obtained from stereological cell counts. The cell counts were done over the whole transgene-expressing region in each section. n = 2 biologically independent animals. See the Methods for the details of fluorescence intensity and cell-count analyses. Source data are provided as a Source data file.

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