Fig. 4: EV-sorting ability of candidate proteins in standardized production conditions.
From: Identification of scaffold proteins for improved endogenous engineering of extracellular vesicles
a Workflow of EV production and SEC fractionation protocol. Created with BioRender.com. b SEC elution profiles of conditioned media from transfected HEK-293T cells. EVs and soluble proteins were recovered in fractions 0–3 and 4–12, respectively. Tluc activity in each fraction was measured with and without Triton and ProK, and normalized to the fraction with the highest signal. c Percentage of intravesicular Tluc using the screening (upper panel; five biological replicates) and standardized (lower panel; four biological replicates) protocols. Mean ± standard deviation. d Calculated Tluc activity per vesicle for purified EV preparations. Results are shown as an average of two biological replicates. Proteins are marked with gene names. Source data are provided as a Source Data file. SEC size exclusion chromatography.
