Fig. 2: Predicted structure and sequence of DEBSM6 and KS-AT and post-AT linker.
From: Biosensor Guided Polyketide Synthases Engineering for Optimization of Domain Exchange Boundaries
a AlphaFold structure prediction of homodimeric DEBSM6 without TE, (b) a highlighted structure of the KS-AT linker in dark red, and (c) the post-AT linker in teal. The regions highlighted are the sequences selected for the junction library. d Alignment of the region selected for the junction library of DEBSM6 and EpoM4 KS-AT and (e) post-AT linker with secondary structure elements predicted by AlphaFold. Domain boundaries marked with blue boxes were predicted using an online tool49, except the start of ψKR which was placed at the beginning of β5. Each junction position in KS-AT linker is sequentially named us1-102 and post-AT junctions called ds1-90. Highly conserved residues are marked with asterisks. DEBSM6 KS-AT linker sequence starts with HV to denote where the conserved GTNAH sequence is positioned. Gaps in alignments are marked in grey. For reference, the position of the first amino acid in the KS-AT sequence is I1908 for DEBSM6 and V1948 for EpoM4 and in the post-AT linker, the first position is A2301 and P2345, respectively.
