Fig. 4: Enzymatic activity (v) plotted against ΔG1 and ΔGT based on Eq. (10).
From: Thermodynamic principle to enhance enzymatic activity using the substrate affinity

The substrate concentration (\([{{{{{\rm{S}}}}}}]\)) in each panel was a 10−1, b 1, c 10, and d 102 \(\mu\)M, as indicated in the bottom right of each panel. In all panels, the black dashed line corresponding to \({K}_{m}=[{{{{{\rm{S}}}}}}]\) overlaps with the region of highest enzyme activity.