Fig. 5: Volcano plots showing how the activity is expected to change with respect to the Michaelis-Menten constant (Km).
From: Thermodynamic principle to enhance enzymatic activity using the substrate affinity
As the substrate concentration was increased from 10-1 \(\mu\)M (black) to 102 \(\mu\)M (red), the volcano plot shifted to the upper right. The apex is located at \({K}_{m}=[{{{{{\rm{S}}}}}}]\), as indicated by the vertical dashed lines of the corresponding color. Changing the values of \(\Delta {G}_{T}\), \({k}_{1}^{0}\) or \({k}_{2}^{0}\) does not influence the conclusion that the activity is maximized when \({K}_{m}=[{{{{{\rm{S}}}}}}]\), as shown in Supplementary Note 3.
