Fig. 1: Enzyme activity and cryo-EM structure of Chs1.
From: Structure, catalysis, chitin transport, and selective inhibition of chitin synthase
a Synthesis and transport scheme of chitin by Chs1. b In vitro UDP-Glo glycosyltransferase assay with purified Chs1 in buffer with (WT) or without (noGlcNAc) GlcNAc. The assay under WT conditions was also performed with EDTA, trypsin, NikkoZ, or PolyB. The reaction without Chs1 was used as the control. Data points represent the mean ± SD in triplicate. Source data are provided as a Source Data file. c In vitro chitin synthesis assay with purified Chs1 in buffer with (WT) or without (noGlcNAc) GlcNAc. The assay under WT conditions was also performed with EDTA and trypsin. The reaction without Chs1 was used as the control. Data points represent the mean ± SD in triplicate. Source data are provided as a Source Data file. d Cryo-EM map and atomic model of the apo Chs1 dimer. e Cartoon representation of the Chs1 monomer. The polypeptide is shown in rainbow from the N- to C-terminus. The putative active site is highlighted by a orange circle. The putative chitin transporting channel is outlined by a purple arrow. f Cartoon topology of the Chs1 monomer. g The Chs1 domain map. Major domains and motifs are labeled. The invisible N-terminal region is in white.
