Fig. 2: Chemogenetic stimulation of olfactory mitral and tufted cells modulates energy expenditure in a sex-specific manner. | Nature Communications

Fig. 2: Chemogenetic stimulation of olfactory mitral and tufted cells modulates energy expenditure in a sex-specific manner.

From: A sex-specific thermogenic neurocircuit induced by predator smell recruiting cholecystokinin neurons in the dorsomedial hypothalamus

Fig. 2

a Schematic of the experiment. Mice were either treated with CNO prior to behavior testing or received saline prior to CNO in indirect calorimetry assays. b Immunostaining of MOB from Tbx21-Cre+-hM3Dq+ animal (Cre+), citrine (green) and cFos (red), scale 300 µm, Two independent experiments, N = 6. c Analysis of cFos positive cells in MOB, males (light blue/blue) and females (pink/red), males, N = 7 per group, females, N = 8/Cre− and N = 6/Cre+, Two-way Anova with Tukey’s post hoc comparison. d Buried pellet test 20 min following CNO injection, males, N = 11/Cre- and N = 6/Cre + , females, N = 9 per group, Two-way Anova with Tukey’s post hoc comparison. e Open field test 20 min following CNO injection, N = 11 per group, Two-way Anova with Tukey’s post hoc comparison. f Levels of corticosterone measured 90 min after CNO injection, males, N = 8 per group, females, N = 7 per group. g, h VO2 for 3 h post-CNO Injection, males, N = 14 per group, females, N = 13 per group, Two-way Anova with Sidak’s post hoc comparison. i, j Food intake for 3 h post-CNO Injection, males, N = 14 per group, females, N = 13 per group. k, l Total activity for 3 h post-CNO Injection, males, N = 14/per group, females, N = 13 p. m, n Refeeding after overnight fasting post-CNO Injection, males, N = 15/Cre− and N = 17/Cre + , females, N = 12/Cre− and N = 10/Cre + . o–q Representative infrared images of ROI selected to measure BAT temperature and analysis of BAT temperature upon CNO injection, males, N = 8/Cre− and N = 11/Cre + , females, N = 9/Cre− and N = 12/Cre + , Three-way Anova. r Plasma free fatty acid levels in mice collected 90 min post CNO delivery, males, N = 8 per group, females, N = 7 per group, Two-way Anova with Tukey’s post hoc comparison. All bar graphs are presented as mean values ± SEM. Table S2 contains the detailed results of the statistical analysis. *p < 0.05, **p < 0.01, ***p < 0.001. Source data are provided as a Source Data file. corticosterone (CORT), Clozapine-N-Oxide (CNO), Brown adipose tissue (BAT).

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