Fig. 5: Effect of predator odor exposure and chemogenetic activation of olfaction on activity of CRH-expressing neurons in PVH and CeA. | Nature Communications

Fig. 5: Effect of predator odor exposure and chemogenetic activation of olfaction on activity of CRH-expressing neurons in PVH and CeA.

From: A sex-specific thermogenic neurocircuit induced by predator smell recruiting cholecystokinin neurons in the dorsomedial hypothalamus

Fig. 5

a Immunohistochemical and in situ hybridization co-staining of Crh (green) and cFos protein (red) in PVH of female mice after exposure to TMT, yellow cells express Crh/cFos, scale 50 µm and 100 µm. b Quantification of neuronal activity in PVH after exposure to TMT, males (light blue/blue) and females (pink/red), N = 5 per group, Two-way Anova with Tukey’s post hoc comparison. c Quantification of the percentage of activated Crh-positive cells in PVH after TMT exposure, N = 5 per group. d Immunohistochemical and in situ hybridization co-staining of Crh (green) and cFos protein (red) in CeA of female mice after exposure to TMT, yellow cells express Crh/cFos, scale 300 µm. e Quantification of neuronal activity in CeA after exposure to TMT, N = 5 per group, Two-way Anova with Tukey’s post hoc comparison. f Quantification of the percentage of activated Crh-positive cells in CeA after TMT exposure, N = 5 per group. g, h Immunohistochemical and in situ hybridization co-staining of Crh (green) and cFos protein (red) in PVH of Tbx21-Cre− and Cre-+-hM3Dq+ female mice after CNO injection, yellow cells express Crh/cFos, scale 50 µm. i, j Quantification of cFos-positive cells in PVH and CeA post CNO injection, N = 5 per group, Two-way Anova with Tukey’s post hoc comparison. k, l Quantification of the percentage of activated Crh-positive cells in PVH and CeA, males, N = 4/Cre- and N = 5/Cre + , females, N = 5 per group. All bar graphs are presented as mean values ± SEM. Table S5 contains the detailed results of the statistical analysis. *p < 0.05, **p < 0.01, ****p < 0.0001. Source data are provided as a Source Data file. 2,4,5-trimethylthiazoline (TMT), paraventricular hypothalamus (PVH), central amygdala (CeA).

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