Fig. 7: Activation of CCK-expressing neurons in DMH upon chemogenetic stimulation of olfaction or predator odor exposure. | Nature Communications

Fig. 7: Activation of CCK-expressing neurons in DMH upon chemogenetic stimulation of olfaction or predator odor exposure.

From: A sex-specific thermogenic neurocircuit induced by predator smell recruiting cholecystokinin neurons in the dorsomedial hypothalamus

Fig. 7

a Representative image of immunohistochemical and in situ hybridization of co-staining for Cck (green) and cFos protein (red) in dDMH and cDMH post-CNO injection, scale 100 µm and 300 µm. b Quantification of the percentage of cFos-positive cells that co-express CCK in dDMH (left) and cDMH (right) post-CNO injection, N = 6 per group, Unpaired t-test,Two-tailed. c Representative image of immunohistochemical and in situ hybridization of co-staining for Cck (green) and cFos protein (red) in dDMH and cDMH after TMT exposure, scale 50 µm and 100 µm. d Quantification of the percentage of cFos-positive cells that co-express CCK in dDMH (left) and cDMH (right) after TMT exposure, N = 4 per group, Unpaired t-test,Two-tailed. Source data are provided as a Source Data file. dorsal dorsomedial hypothalamus (dDMH) and compact dorsomedial hypothalamus (cDMH), clozapine-N-Oxide (CNO), 2,4,5-trimethylthiazoline (TMT).

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