Fig. 5: HSAL mediated increase of iron bioavailability is comparable to that mediated by EDTA. | Nature Communications

Fig. 5: HSAL mediated increase of iron bioavailability is comparable to that mediated by EDTA.

From: A lignin-derived material improves plant nutrient bioavailability and growth through its metal chelating capacity

Fig. 5

a Representative images showing the effect of 0.05% HSAL on the recovery of iron deficiency phenotype in different Arabidopsis iron uptake defective mutants. Seedlings of wild-type Col-0 and mutants opt3-2, irt1−1 and fro2 were grown under ½ MS medium (+Fe) and iron-deficient medium (-Fe) for 10 days. b Total chlorophyll content. Graphs depict mean with standard deviation (error bars) and individual data points (n = 3 biologically independent replicates). c Box plots and scatter plots showing root length. Boxes represent the 25th–75th percentile range, white lines represent medians and whiskers show the minimum–maximum range. Different letters indicate statistically significant differences based on one-way ANOVA and Tukey’s HSD test analysis (p <  0.05) in b–e Effect of different concentrations of EDTA-Na2 (0–1300 μM) on the recovery of iron deficiency phenotypes in wild-type Arabidopsis and different iron uptake and transport defective mutants (opt3-2, irt1−1and fro2). The seedlings were grown on iron-sufficient medium (½ MS with 50 μM EDTA-Fe (III), pH = 5.7) or iron-deficient medium (½ MS without EDTA-Fe (III), pH = 5.7) for 9 days. Total chlorophyll content under iron deficiency d and iron sufficient conditions e. N = 4 biologically independent replicates. f, g Root length under iron deficiency f and iron sufficient conditions g. The lines indicate mean and standard deviation (error bars). N = 15 seedlings. Different letters indicate statistically significant differences among genotypes d–g based on one-way ANOVA and Tukey’s HSD test analysis (p <  0.05).

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